Protein and Enzyme Biology

Lab Syllabus

Fall 2005

Project #1

P1        Introduction to the lab                                               Lab #1             August 30, 2005

1.     Mac Help

2.      Learning Cricket graph

3.      Pipets and Pipetman

4.      pH meter

4.      Getting solutions ready for next lab

P2        Methods of protein determination                          Lab #2             September 1, 2005

P3        Enzyme kinetics                                                            Lab #3             September 6, 2005

Project #2

P1        Coupled enzyme assay                                              Lab #4

P2        Tissue extraction and protein precipitation           Lab #5

P3        Specific activity                                                             Lab #6

P4        Purification affinity chromatography                      Lab #7

P5        Gel electrophoresis                                                       Lab #8 and 9

Project #3

Characterization of GAPDH                                         Lab #10

           Gel chromatography