1) They should allow you to reconstruct what you did during the experiment.
This means you should record things like amplifier, stimulator settings, and the times at which experiments were done (even if they are also in a computer file). Index all of your computer files and pages in the notebook, so that you know where they fit in the experiment (write down the file names and the directory in which they are saved). Try to be organized, so that if you had to reconstruct what you did you could do so easily, without having to blindly search through your lab books. Changes from the lab manual procedures should also be noted, as well as details of how things went not included in the basic procedure. Make detailed drawings of how your dissection went and where you made your recordings (such as left vs. right side, segment #). In order to make them detailed, you will need to make them large. Fill the whole page.
Note times, electrode changes & resistances, saline
changes, and anything
else that could affect the experiement.
One should use pen, not pencil, so the
writing actually reflects what happens during the lab including any
false starts or
mistakes. Don’t worry about cross-outs, just make sure everything
is
clear.
2) They should contain your results and comments on the significance of your results.
Besides just writing down numbers, you should say something
about what the numbers mean. For example, you may have found that
action potential
latencies in the sciatic nerve decreased when you switched the polarity
of
the stimulating electrodes from having the positive pole nearer the
recording
electrodes to having the negative pole nearer the recording
electrodes.
Not only should you write down the values for the latencies (use only significant
digits), you should say what the result might mean (that the action
potential
may be generated at a site closer to the negative stimulating
electrode). If you are not sure what a result means, please
ask. Don’t just mechanically work through the lab exercise; think
about what you are seeing, and let your
lab notebook show that you have given your results some thought.
3) Graphics.
Use sketches to illustrate your experimental set-up, to show how the dissection works, and to show how your recordings were made. When using graphics to illustrate data, be sure to label each axis and include units. When using computer generated graphics, be sure the graph is smaller than your notebook page, and paste or tape it into your notebook without folding it.
Grading: Each week’s lab notebook will be worth 10 points (100 total points ).
The first grade will be dropped and won’t count towards the final grade.
Other advice:
Write in the lab notebook as you go along; don’t try to write
up the
experiments after you’ve done them. In general, it is better to
write
more than less. It is helpful to tape or glue computer printouts
and
graphics into your notebooks to illustrate results. Print them
small
enough that they don't need to be folded. Tables of contents are
unnecessary.
modified 8-22-04