Pyruvate kinase is one of the most important control steps in glycolysis, along with phosphofructokinase and hexokinase. It is activated by AMP and is inhibited by ATP and acetyl coA. In the last step of glycolysis, once phosphoenolpyruvate (pep) has been converted into pyruvate it cannot go back to pep, this is called a committed step. If this step happens under conditions with plenty of oxygen, the pyruvate will be turned in to CO2. If this step is prevented then pep is used in gluconeogenesis instead. Two ATP’s are made from the transformation of pep to pyruvate using pyruvate kinase. Those pyruvates will either be fermented into alcohol, turned into lactate, or be put through the pyruvate dehydrogenase complex and form acetyl coA and NADH.